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What is RT PCR? |Reverse Transcriptase PCR

What is RT PCR? |Reverse Transcriptase PCR
What is RT PCR

RT PCR or Reverse Transcriptase PCR is a test used to detect retroviral diseases like AIDS. It gained popularity during coronavirus outbreak.

Before knowing about RT PCR, please refer PCR or Polymerase chain reaction first here – https://sciencebirdie.com/2020/06/17/what-is-pcr-polymerase-chain-reaction-explained/

PCR or polymerase chain reaction is the technique in which the DNA is amplified and the billion DNA copies are made. But, it has exceptions like it can only detect only DNA viruses. Therefore, detection of RNA viruses like retroviruses cannot be possible.

What is RT PCR

This is where the RT PCR has the significance.

Working mechanism

Viruses like Coronavirus have RNA as the genetic material, therefore, the detection using the traditional PCR becomes impossible.

So, scientists convert RNA to DNA using an enzyme called Reverse transcriptase, the process known as Reverse Transcription. On the other hand, the conversion of DNA to RNA is called Transcription.

What is RT PCR
Thermocycler or PCR machine used in PCR technique

Steps in RT PCR

Firstly, the RNA converted to DNA using reverse transcription.

The DNA then transferred to the thermocycler machine or the PCR machine.

Afterwards, the steps denaturation, annealing and extension followed and the billion DNA copies extracted.

Please refer the PCR steps here – https://sciencebirdie.com/2020/06/17/what-is-pcr-polymerase-chain-reaction-explained/

Advantages

Coming to advantages, it had got more in addition than the traditional PCR.

Highly sensitive as the RNA involvement in it.

Highly specific, with more accuracy than PCR.

Requires less than 4 hours in diagnosis, moreover, the laboratories take up to 6-8 hours.

As the genetic material placed in a closed tube with enzymes, it has low potential contamination.

RT PCR in Corona test

Nowadays, with Coronavirus being prevalent, the RT PCR use got very much boosted up.

What is RT PCR

Firstly, the victim’s blood sample is taken then using several enzymes the RNA is extracted and converted to DNA and with PCR many copies are made.

Now, the DNA bases are made to pair with another bases tagged with markers. Further, the pairs are examined through fluorescence technique. If there are abnormalities, in the pairs the pairing does not occur. In other words, the person stated positive in the test.

So, folks tell me! Do you like my works, please comment down and let me know. Also, share with your near and dear ones. Let us build a strong science community.

Cheers!

Increase immunity during coronavirus outbreak traditionally

Increase immunity during coronavirus outbreak traditionally

Increase immunity during coronavirus outbreak traditionally.

The world is suffering with the global pandemic, the coronavirus. The reason for the high effect is that the virus can affect 2 to 2.5 people by a carrier person. Also, the virus being undetectable with the symptoms showing in 14 days.

The world together continues to fight it with vaccine research and trials going on.

We do not have any option for medicines. So, in these days we can remain isolated and practice measures to increase immunity. Because left with no vaccines and antidote we only have immunity. It all depends on immunity now !

So, here we present easy traditional ways through which you can power up your immune cells. Increase immunity during coronavirus outbreak traditionally

Here are some of the tips:

Ginger(Zingiber officinale)

Use ginger( Zingiber officinale ), as it increases immunity. Also it eases cold and flu so it is an effective way to fight the coronavirus.

Increase immunity during coronavirus outbreak traditionally

You can consume ginger by smashing it and consuming it with honey. Honey is also better in powering up immunity. It decreases cough. Or you can use dry ginger powder with honey.

Increase immunity during coronavirus outbreak traditionally

Tulsi(Ocimum sanctum)

Tulsi( Ocimum sanctum ) is very beneficial. Use tulsi leaves by taking in with boiled water.

Increase immunity during coronavirus outbreak traditionally

Boil water by adding tulsi( Ocimum sanctum) in it. Also you can add ginger in it and consume.

Turmeric(Curcuma longa)

Use turmeric powder in food as it has anti fungal and anti bacterial properties. So it reduces infection.

 turmeric powder Increase immunity during coronavirus outbreak traditionally

It can be used in boiled water by adding a whole spoon of it.

So, these are the traditional methods to make your immunity healthy. I have been practicing it and found it effective. This ayurvedic traditional method is indeed powerful.

Also, do not use antibiotics in any case, it will only lower your immunity and will not act against the coronavirus.

So, use this method and comment down your experience with these traditional tips!

For learning more about Ayurveda visithttp://anahataayurveda.in/

Top facts of new coronavirus china

coronavirus china

Top facts of new coronavirus china

The new coronavirus impact is huge.

Started in Wuhan province of China.It created impact worldwide.

Here are the top facts of new coronavirus china:

Official name of the virus

Official name is assigned to the virus. There are different strains of coronavirus. They may differ in various aspects.Officially the name of the virus is severe acute respiratory syndrome coronavirus 2 
(SARS-CoV-2)
given according to the act of virus.It severely induced acute respiratory syndrome.

Official name of the disease

There are lot of differences between the disease and virus. Likewise, the name of the disease is COVID-19.Where, CO stands for corona,VI for virus,D for disease and 19 stands for the year 2019 in which the outbreak started.

COVID19

NO TREATMENT YET!

Still no treatment for the virus found.Various experiments conducted across the world showed no fruitful message till yet.

Early spread

Earlier an outbreak of coronavirus in the Middle east took place .

Therefore, named as MERS(Middle East respiratory syndrome).Believed to spread from camels,still unclear based on reports.

Spread facts of new coronavirus china

The spread started from a live animal market.Therefore,the government banned the transport of live animals.Sea foods strictly discouraged to consume.

live animal market

In contrast the MERS case and SARS case are similar and highlight one query, How the transmission of the virus takes place from animals to humans?

MERS VS SARS

According to the present condition and analysis.The SARS-COV-2 is more contagious.SARS infected patients require more care than MERS one.SARS case is more complicated.

CORONAVIRUS MYTHBUSTERS

Corona Virus

coronavirus mythbusters

Coronavirus is a serious issue of the world.China is suffering a lot.Lot of myths circulated nowadays regarding the disease.

Here we present the coronavirus mythbusters

Chinese packages are not safe

Coronavirus do not survive long in packages or objects.The WHO(World health organization) analyzed and confirmed.Therefore,people in contact with package do not contract the coronavirus.

Coronavirus spread through pets

No evidence present regarding this issue.But practice hygiene.

Frequently wash hands after touching the pets.Get the pets vaccinated.In addition keep the pets clean.

Antibiotics effective for the treatment?

Antibiotics only work for bacteria.Not effective for virus.Antibiotics do not treat viral infections.The new corona virus is a virus.

However,hospitalized persons may receive the antibiotics.Because a bacterial infection chance is there.

Medicines for the coronavirus

Till date no medicines or treatment for the virus.But infected person needs good care and hospitalization.

People die once contracted

Only 2% cases faced deaths because of weak immunity.People with high immunity recover in a short period of time.But great care should be followed.