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What is RT PCR? |Reverse Transcriptase PCR

What is RT PCR? |Reverse Transcriptase PCR
What is RT PCR

RT PCR or Reverse Transcriptase PCR is a test used to detect retroviral diseases like AIDS. It gained popularity during coronavirus outbreak.

Before knowing about RT PCR, please refer PCR or Polymerase chain reaction first here – https://sciencebirdie.com/2020/06/17/what-is-pcr-polymerase-chain-reaction-explained/

PCR or polymerase chain reaction is the technique in which the DNA is amplified and the billion DNA copies are made. But, it has exceptions like it can only detect only DNA viruses. Therefore, detection of RNA viruses like retroviruses cannot be possible.

What is RT PCR

This is where the RT PCR has the significance.

Working mechanism

Viruses like Coronavirus have RNA as the genetic material, therefore, the detection using the traditional PCR becomes impossible.

So, scientists convert RNA to DNA using an enzyme called Reverse transcriptase, the process known as Reverse Transcription. On the other hand, the conversion of DNA to RNA is called Transcription.

What is RT PCR
Thermocycler or PCR machine used in PCR technique

Steps in RT PCR

Firstly, the RNA converted to DNA using reverse transcription.

The DNA then transferred to the thermocycler machine or the PCR machine.

Afterwards, the steps denaturation, annealing and extension followed and the billion DNA copies extracted.

Please refer the PCR steps here – https://sciencebirdie.com/2020/06/17/what-is-pcr-polymerase-chain-reaction-explained/

Advantages

Coming to advantages, it had got more in addition than the traditional PCR.

Highly sensitive as the RNA involvement in it.

Highly specific, with more accuracy than PCR.

Requires less than 4 hours in diagnosis, moreover, the laboratories take up to 6-8 hours.

As the genetic material placed in a closed tube with enzymes, it has low potential contamination.

RT PCR in Corona test

Nowadays, with Coronavirus being prevalent, the RT PCR use got very much boosted up.

What is RT PCR

Firstly, the victim’s blood sample is taken then using several enzymes the RNA is extracted and converted to DNA and with PCR many copies are made.

Now, the DNA bases are made to pair with another bases tagged with markers. Further, the pairs are examined through fluorescence technique. If there are abnormalities, in the pairs the pairing does not occur. In other words, the person stated positive in the test.

So, folks tell me! Do you like my works, please comment down and let me know. Also, share with your near and dear ones. Let us build a strong science community.

Cheers!

FIRST 3D MAP OF CORONAVIRUS TO DEVELOP VACCINES

FIRST 3D MAP OF CORONAVIRUS TO DEVELOP VACCINES
FIRST 3D MAP OF CORONAVIRUS TO DEVELOP VACCINES

FIRST 3D MAP OF CORONAVIRUS TO DEVELOP VACCINES

  • Researchers of University of Texas and National Institute of Health successfully created the first 3D map of the novel coronavirus.
  • Created within 27 days
  • Obtained viral genome sequence in 15 days
  • Constructed 3D map of the novel coronavirus in 12 days
FIRST 3D MAP OF CORONAVIRUS TO DEVELOP VACCINES

Index

FIRST 3D MAP OF CORONAVIRUS TO DEVELOP VACCINES

Researchers of University of Texas, Austin and National Institute of Health, U S successfully created the first 3D map of the novel coronavirus.

The three dimensional atomic scale map will help majorly in developing vaccines. Also antiviral drugs to resist the viral attacks.

In a due course of 15 days the scientists obtained the viral genomic sequence of the nove coronavirus.

In further 12 days the team reconstructed the 3D map of novel coronavirus.

What is 3D mapping?

 3D mapping of brain
This is the 3D mapping of brain used to study in further advancements

3D mapping is the modern technique which is a part of Augmented Reality(AR).

The objects are designed and projected using a projector, mainly used in displaying architectural aspects, designs.

Used in theatres mainly in entertainment. Above all, used in biological aspects to display the human body anatomically for case studies. Also in Genome sequencing.

In addition, using 3D mapping we can recreate or construct anything. Therefore it is required and emerging in all the fields. This piece of technology can prove it’s excellence in coming days.

Use of the 3D map of novel coronavirus

FIRST 3D MAP OF CORONAVIRUS TO DEVELOP VACCINES

The map of the viral protein structure of novel coronavirus revealed the difference of the behavious patterns of it’s strains.

Therefore, It helped in comparison of novel coronavirus with MERS and SARS. Consequently, it showed how this virus spreads faster. In contrast , between the other two.

Expected to help in creating the first vaccine. Furthermore, it can help in studying the viral effects.

Also, it can help in preparation from another outbreak.

15 years ago the human genome was sequenced. Likewise, the sequencing and 3D mapping can help to know more about the microworld.

What can you expect from 3D mapping technology? Comment and let me know!

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CORONAVIRUS CHINA

by sciencebirdie 2 Comments

CORONAVIRUS CHINA

A new virus related to SARS is behind China's mysterious pneumonia outbreak #worldnews

CORONAVIRUS CHINA LATEST SPREAD

CORONAVIRUSs CHINA.Recently, virus spread is rapid in China.The spread started from Wuhan district.106 people died due to the infection.

Spreading started from sea food market.As a result the government advised to prevent the sea food use and consume.

Currently, no public transport is available in 10 cities.Also,airports and railways closed.

SPREAD IN INDIA

Three cities in India are on high alert.Mumbai,Delhi and Kolkota are on high alert.

2 people out of 6 suspected because they showed some symptoms.Thermal scanning conducted in airports.

Recently,10 arrived people’s samples sent to medical labs.Gladly,the results came negative.The contagious virus can spread rapidly.Dry body conditions can create vulnerable conditions.

CORONAVIRUS SYMPTOMS

Person after infection can suffer acute respiratory syndrome.Diseases more related to respiration.

Above all,Symptoms include sore throat and cough and cold.Headache is considerably noted.

CORONAVIRUS PREVENTION

12 Things You Should Do Everyday - Drink Lots Of Water - www.Everythingabode.com-glass of water with white background

No treatment or antidote for the corona virus.Once infected conditions can be lethal.But preventive measures can be taken.

Highly advised to wear masks in public places.Highly recommended to drink water.Because dry throat is the best breeding ground for virus.

Therefore,Keep the mouth and throat moist.